Answer: c - His father's mother cannot roll her tongue.
Explanation: His father must have inherited the trait from his grandfather. This means that, the genotype of the grandfather can either be Homozygous (RR) or heterozygous (Rr) for the trait while the genotype of the grandmother can only be rr since she can't roll her tongue.
Crossing RR with rr (possible genotypes of his paternal grandparents), the probability that his father will be heterozygous for the trait is ½.
Crossing Rr with rr (another possible genotypes of his paternal grandparents), the probability that his father will be heterozygous for the trait is 1.
In either way, one can establish the heterozygosity of his father only if his father's mother cannot roll her tongue.