Match the following items,
1. obtaining DNA for analysis from blood or other body fluid
quantification
using heat or chemicals to break open the cells and free the
DNA
purification of the sample
separation of cellular debris and other impurities from the
DNA
gel electrophoresis to separate
DNA fragments
sample collection
4. determination if the sample is sufficient for analysis
5. copying the DNA using the polymerase chain reaction
DNA extraction
6. electrically ordering the fragments
amplification using PCR

Question

madisonhayes1974 madisonhayes1974

02-03-2020
Biology

contestada

Match the following items,
1. obtaining DNA for analysis from blood or other body fluid
quantification
using heat or chemicals to break open the cells and free the
DNA
purification of the sample
separation of cellular debris and other impurities from the
DNA
gel electrophoresis to separate
DNA fragments
sample collection
4. determination if the sample is sufficient for analysis
5. copying the DNA using the polymerase chain reaction
DNA extraction
6. electrically ordering the fragments
amplification using PCR

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monica789412 monica789412 · Answer 1 · 2020-03-10T12:09:37+01:00

1. Obtaining DNA for analysis from blood or other body fluid -sample collection.

Using heat or chemicals to break open the cells and free the DNA - DNA extraction.

Separation of cellular debris and other impurities from the DNA- purification of the sample

4. Determination if the sample is sufficient for analysis -- quantification

5. Copying the DNA using the polymerase chain reaction-- electrically ordering the fragments amplification using PCR

Explanation:

These are the processes involve in extraction and amplification of DNA sample.

Quantification is done to know if the sample is enough for the whole process of DNA extraction to occur.

DNA sample can be obtained from blood or other body fluids as viscera or semen.

The DNA from the sample is extracted and make free from other cellular components. The lysis of the cell takes place by heat to let the nucleus broken and free the DNA in the cell.

Precipitation: The DNA is purified and precipitated in the ethanol solution as it is insoluble in ethanol.

By gel electrophoresis the fragments of DNA obtained will be sequenced.

The DNA obtained is amplified using polymerase chain reaction.

mickymike92 mickymike92 · Answer 2 · 2022-01-27T12:23:44+01:00

Based on the steps of DNA purification, the correct matches are:

Obtaining DNA for analysis from blood or other body fluid - Sample collection.
Using heat or chemicals to break open the cells and free the DNA - DNA extraction.
Separation of cellular debris and other impurities from the DNA- purification of the sample
Determination if the sample is sufficient for analysis -- quantification
Copying the DNA using the polymerase chain reaction-- electrically ordering the fragments amplification using PCR

What are the steps in DNA isolation and purification?

The steps in DNA isolation and purification are as follows:

Sample collection
DNA extraction
Purification of sample
Quantification of DNA extracted
PCR amplification of DNA

Step 1: Sample collection: this involves obtaining DNA for analysis from blood or other body fluid.

Step 2: DNA extraction- this involves using heat or chemicals to break open the cells and free the DNA.

Step 3: Purification of the sample- this involves separation of cellular debris and other impurities from the DNA.

Step 4: Quantification- Determination if the sample is sufficient for analysis.

Step 5: Amplification using PCR- this involves copying the DNA using the polymerase chain reaction.

Learn more about DNA extraction and purification at: https://brainly.com/question/15294154